CLARIOstar
The CLARIOstar is a multi-mode microplate reader with advanced LVF Monochromators™, highly sensitive filters, and an ultra-fast UV/vis spectrometer. It is a modular microplate reader with up to seven different detection modes: fluorescence intensity (incl. FRET), fluorescence polarization, luminescence (including BRET), UV/vis absorbance, time-resolved fluorescence, TR-FRET, and AlphaScreen®/AlphaLISA®/AlphaPlex™.
Features
- Innovative monochromators for increased sensitivity
- Fully-flexible wavelengths and bandwidths
- Spectral scanning in AS, FI and LUM
- Higher light transmission
- High-speed absorbance measurements
- Dedicated laser for Alpha Technology
- Live cell-based assays
- Low volume measurements
CLARIOstar
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Detection Modes
Fluorescence intensity (incl. FRET)
Fluorescence polarization
AlphaScreen®/AlphaLISA®/AlphaPlexTM
Luminescence (flash and glow) - incl. BRET
Time-resolved fluorescence
TR-FRET
UV/Vis absorbance spectra
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Measurement Modes
Top and bottom reading
Endpoint and kinetic
Sequential multi-excitation
Sequential multi-emission
Spectral scanning (fluorescence, luminescence, absorbance)
Ratiometric measurements
Well scanning
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Microplate Formats
6- to 1536-well plates, user-definable
LVis Plate with 16 low volume microspots (2 μL)
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Microplate Carrier
Robot compatible
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Light Sources
High energy xenon flash lamp
Dedicated laser for AlphaScreen®/AlphaLISA®/AlphaPlexTM
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Detectors
Low-noise photomultiplier tube
CCD spectrometer
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Wavelength Selection
Dual Linear Variable Filter (LVF) Monochromators™
Linear Variable Dichroic Mirror
Separates ex & em LVF Monochromators
Optical filters:
Ex and em slides hold 4 filters each
LVF Monochromators + optical filters
Use one for ex and the other for em
UV/Vis absorbance spectrometer:
Full spectra or 8 distinct wavelengths in < 1 sec/well
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Optical Filters
Excitation and emission slides for 4 filters each
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Optical Path Guides
Top and bottom: enclosed, free-air optical light path guided by motor-driven mirrors and dichroics
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Z-Adjustment
Automatic focal height adjustment (0.1 mm resolution)
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Spectral Range
Filters 240 - 750 nm or 240 - 900 nm for FI, FP, TRF
240 - 750 nm for LUMLVF Monochromators™ 320 - 850 nm for FI
320 - 750 nm for LUMLinear Variable Dichroic 340 - 740 nm for FI, LUM Spectrometer 220 - 1000 nm for ABS
