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    PHERAstar FSX

PHERAstar FSX

The most sensitive high-throughput screening plate reader on the market

The PHERAstar® FSX is our latest, most sensitive multi-mode reader for high-throughput screening, combining this sensitivity with fast read times. Thanks to the unique Optic Module system, Simultaneous Dual Emission, UV/Vis spectrometer, and dedicated AlphaScreen® and TRF Lasers, whatever your application, the PHERAstar FSX will perform it with ease and speed, even in 3456-well microplates.

The new gold standard for High-Throughput Screening.

 

Details

Never worry about what dichroic or filter is installed in your PHERAstar FSX. Easy-to-handle, assay-optimized Optic Modules contain application-specific filters, mirrors, dichroics, and/or polarizers, and are automatically recognized by the reader. In addition, the PHERAstar FSX is equipped with four matched and assay-optimized photomultiplier tubes (PMTs) that are automatically selected for each detection mode, as well as for Simultaneous Dual Emission

For HTS automation purposes, the PHERAstar FSX offers improved robotic integration capabilities, multi-user control and MARS data analysis software with included digital signature and FDA 21 CFR part 11 compliance, and can be equipped with BMG LABTECH's Stacker.

Features

  • The most sensitive reader in Fluorescence Intensity and Polarization
  • Fastest read times with Simultaneous Dual Emission, including for Alpha Technology
  • 9 decades luminescence dynamic range
  • Full absorbance spectra from 220-1000 nm in less than 1 second per well
  • Top and bottom reading with focal height adjustment (0.1 mm z-height)
  • All microplate formats up to 3456-well
  • New generation TRF laser for highest performance
  • Dedicated AlphaScreen®/AlphaLISA®/AlphaPlexTM laser
  • Advanced well scanning with up to 900 data points per well
  • Two onboard, high-precision injectors with simultaneous reagent injection and detection
  • Includes multi-user control and MARS data analysis software
  • Stacker and robot compatibility

 

Detection Modes

Fluorescence Intensity

FRET

Fluorescence Polarization

AlphaScreen®/AlphaLISA®/AlphaPlexTM

Luminescence (flash and glow)

BRET

Time-Resolved Fluorescence - including TR-FRET

UV/Vis absorbance spectra

Measurement Modes

Top and bottom reading

Endpoint and kinetic measurements

Sequential multi-excitation measurements

Sequential multi-emission measurements

Simultaneous Dual Emission measurements

Spectral scanning (absorbance)

Real-time ratiometric measurements

Well scanning

Microplate Formats

Up to 3456-well plates, user-definable
LVis Plate with 16 low volume microspots (2 μL)

Microplate Carrier

Robot compatible

Light Sources

High energy xenon flash lamp

Dedicated laser for AlphaScreen®/AlphaLISA®/AlphaPlexTM

Dedicated laser for TRF and TR-FRET

Detectors

Two matched pairs of photomultiplier tubes, optimized for different detection modes 
CCD spectrometer

Wavelength Selection

Optic Modules: Up to six application-specific modules (includes all filters, dichroics, etc. for an application) 
For top and bottom reading
UV/Vis Absorbance Spectrometer: 
Full spectra or 8 distinct wavelengths in < 1 sec / well

Optical Path Guides

Top and Bottom: Enclosed, free-air optical light path guided by motor-driven mirrors and dichroics

Z-Adjustment

Automatic focal height adjustment (0.1 mm resolution)

Spectral Range

Filters 230 - 750 nm or 230 - 900 nm for FI, FP 
230 - 750 nm for LUM 
230 - 900 nm for TRF
Spectrometer 220 - 1000 nm for ABS

Sensitivity*

FI
(top)
< 0.15 pM Fluorescein (384sv black, 20 μL)
< 0.5 pM Fluorescein (1536 black, 8 μL)
FI
(bottom)
< 1.0 pM Fluorescein (384g black, 50 μL)
FP < 0.5 mP SD at 1 nM Fluorescein (384sv black, 20 μL)
< 1.5 mP SD at 1 nM Fluorescein (1536 black, 8 μL) 
TRF
with laser
< 5 fM Europium (384, 80 μL)
< 15 fM Europium (1536, 8 μL)  
HTRF®
(black and white microplates)
Reader Control Kit (Eu) after 18h (384sv, 20 μL) 
Delta F > 1100 % (High Calibrator) 
Delta F > 25 % (Low Calibrator)
LUM < 0.4 pM (< 8 amol/well ATP, 384sv white, 20 μL)
Dynamic Range: 9 decades 
AlphaScreen®
with Laser
< 5 pM (< 100 amol/well P-Tyr-100, 384sv white, 20 μL)
ABS
with Spectrometer
Full spectrum captured in < 1 s / well 
Selectable spectral resolution: 1, 2, 5, and 10 nm 
OD range: 0 - 4 OD 
Accuracy: < 1% at 2 OD 
Precision: < 0.5% at 1 OD and < 0.8% at 2 OD

Read Times

Flying mode
(1 flash) 

14 s (384)
27 s (1536) 

10 flashes 38 s (384)
1 min 52 s (1536) 
50 flashes 1 min 29 s (384)
5 min 18 s (1536) 

Reagent Injection

Up to 2 built-in reagent injectors

Injection at measurement position (6 to 384-well)

Individual injection volumes for each well (3 to 500 µL, optionally up to 2 mL)

Variable injection speed up to 420 µL / s

Up to four injection events per well

Reagent back flushing

Shaking

Linear, orbital, and double-orbital with user-definable time and speed

Integrated Barcode Reader

Up to three integrated barcode readers

Incubation

+5 °C above ambient up to 45 °C 

The upper heating plate of the incubation chamber operates at 0.5 °C more than the lower plate. This prevents condensation build-up on the lid or sealer.

Software

Multi-user Reader Control and MARS Data Analysis Software included 
FDA 21 CFR Part 11 compliant

Dimensions

Width: 45 cm, depth: 51 cm, height: 47 cm; weight: 49 kg
 

Optional Accessories

LVis Plate

Sample Capacity: Sixteen separate microdrop wells for 2 µL samples; One standard cuvette position for up to 1 mL samples.

 

Quality Control Internal Standards (optional): Four NIST traceable optical density filters (approximate values of 0.1, 0.3, 0.6 and 1.0 OD); One holmium oxide filter for wavelength accuracy.

 

Dimensions: Conforms to SBS standards for microplates.

Stacker

Magazines for up to 50 plates - continuous loading feature

THERMOstar

Microplate Incubator and Shaker

Upgrades

Please contact your local representative for upgrades including options such as detection modes, reagent injectors, etc.

*

LOD = 3 x SD (20 blanks) / slope (6 pt std curve)

AlphaScreen® P-Tyr-100 assay kit, PerkinElmer, #6760620C

384sv = 384-well small volume microplates

384g = 384-well glass bottom microplates

1536 = 1536-well microplates

  • DLR™ Assay System
  • HTRF® - Homogenous Time-Resolved Fluorescence
  • LanthaScreen® Technology
  • Transcreener® ADP2 FI Assay
  • Transcreener® ADP2 FP Assay
  • Transcreener® ADP2 TR-FRET Assay
  • MycoAlert

 

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